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nature portfolio | rep orting sum m ary m arch 2021 validation rabbit monoclonal antibody recognizing atf4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc nature portfolio | rep orting sum m ary m arch 2021 validation rabbit monoclonal antibody recognizing atf4
    Nature Portfolio | Rep Orting Sum M Ary M Arch 2021 Validation Rabbit Monoclonal Antibody Recognizing Atf4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1686 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m+arch+4/ATF-4+Rabbit+mAb/pmc09573865__41467_2022_33859_MOESM3_ESM-22-1-34
    Average 98 stars, based on 1686 article reviews
    nature portfolio | rep orting sum m ary m arch 2021 validation rabbit monoclonal antibody recognizing atf4 - by Bioz Stars, 2026-09
    98/100 stars

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    Membrane:

    Article Title: Cell death triggered by Yersinia enterocolitica identifies processing of the proinflammatory signal adapter MyD88 as a general event in the execution of apoptosis.
    Article Snippet: .. For the detection of MyD88, the membrane was probed with rabbit polyclonal Ab directed against the C terminus of human MyD88 (recognizing amino acids 279–296; Sigma-Aldrich, Munich, Germany; or a region around amino acid 233; Cell Signaling Technology, Danvers, MA). at U niv of L aval B iblio/Sec D es A cquisitions on M arch 4, 2015 http://w w w .jim m unol.org/ D ow nloaded from Alternatively, myc-tagged overexpressed MyD88 was labeled by probing with a monoclonal mouse anti-myc epitope tag Ab (Life Technologies). .. Immunoreactive bands were visualized using appropriate secondary Abs and ECL detection reagents (GE Healthcare, Bucks, U.K.).

    Labeling:

    Article Title: Cell death triggered by Yersinia enterocolitica identifies processing of the proinflammatory signal adapter MyD88 as a general event in the execution of apoptosis.
    Article Snippet: .. For the detection of MyD88, the membrane was probed with rabbit polyclonal Ab directed against the C terminus of human MyD88 (recognizing amino acids 279–296; Sigma-Aldrich, Munich, Germany; or a region around amino acid 233; Cell Signaling Technology, Danvers, MA). at U niv of L aval B iblio/Sec D es A cquisitions on M arch 4, 2015 http://w w w .jim m unol.org/ D ow nloaded from Alternatively, myc-tagged overexpressed MyD88 was labeled by probing with a monoclonal mouse anti-myc epitope tag Ab (Life Technologies). .. Immunoreactive bands were visualized using appropriate secondary Abs and ECL detection reagents (GE Healthcare, Bucks, U.K.).

    Immunostaining:

    Article Title: Loss of Syndecan-1 Induces a Pro-inflammatory Phenotype in Endothelial Cells with a Dysregulated Response to Atheroprotective Flow
    Article Snippet: .. B, the volume of S1KO cells is reduced compared with the WT model. 9548 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 14 • APRIL 4, 2014 at G eorgian C ourt C ollege on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from antibodies were used for immunostaining: phospho-Akt (Ser473) and Akt from Cell Signaling; phosphopaxillin (Tyr118) from Invitrogen; paxillin from BD Biosciences; and secondary antibodies from Santa Cruz Biotechnology. ..

    Control:

    Article Title: The Cyp2c44 Epoxygenase Regulates Epithelial Sodium Channel Activity and the Blood Pressure Responses to Increased Dietary Salt
    Article Snippet: After sonication (six pulses, power 4) (Ultrasonic Homogenizer 4710; Cole Parmer Instruments), the homogenates were centrifuged for 15 min at 5,000 and 10,000 g. Membrane fractions were collected from 10,000 g supernatants by a 60-min centrifugation at 100,000 g, suspended in fresh homogenization buffer, and used within the next 4 h. Western blot analyses of kidney homogenates or membranes were performed by standard electrophoretic/blotting techniques using the following rabbit peptide antibodies: anti-Cyp2c44 from GenScript; anti-phospho-ERK1/2 (Tyr202/204), anti-phosphothreonine from Cell Signaling Technologies; anti-ERK1/2 and anti-ENaC from Santa Cruz Biotechnology; and anti- -actin from Sigma. .. Control experiments showed that only lot 16 of the anti-phosphothreonine antibod- 4378 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 7 • FEBRUARY 14, 2014 by guest on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from ies purchased from Cell Signaling Technologies recognized protein(s) with the electrophoretic mobility reported for the native channel subunit (75– 80 kDa) (30) and immunoreactive toward anti-ENaC antibodies. .. Immunoreactive proteins were detected with an Immobilon Western chemiluminescent HRP substrate kit (Millipore) and quantified by x-ray silver grain densitometry.

    Article Title: AMPylation of Rho GTPases Subverts Multiple Host Signaling Processes
    Article Snippet: .. Switch-1 AMPylation Blocks Novel Rac Interactions—Although the above findings support the notion that AMPylation of Rho GTPases perturb cell signaling beyond a simple loss of actin cytoskeleton control, we further hypothesized that there may be additional unknown roles of Rho GTPases that might be 32982 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 47 • NOVEMBER 21, 2014 at Y ork U niversity - O C U L on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from disrupted by switch-1 modification. ..

    Modification:

    Article Title: AMPylation of Rho GTPases Subverts Multiple Host Signaling Processes
    Article Snippet: .. Switch-1 AMPylation Blocks Novel Rac Interactions—Although the above findings support the notion that AMPylation of Rho GTPases perturb cell signaling beyond a simple loss of actin cytoskeleton control, we further hypothesized that there may be additional unknown roles of Rho GTPases that might be 32982 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME 289 • NUMBER 47 • NOVEMBER 21, 2014 at Y ork U niversity - O C U L on M arch 4, 2015 http://w w w .jbc.org/ D ow nloaded from disrupted by switch-1 modification. ..



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